| | 1 | = LhARA radiobiology; meeting: 14May26; 14:00 GMT = |
| | 2 | |
| | 3 | **ZOOM:** https://imperial-ac-uk.zoom.us/j/98220889714?pwd=SM90vOF7BKSXoU3mD9q7OwBQo4IVB3.1 |
| | 4 | |
| | 5 | Attended: PH, JMcG, MB, TP, CD, (KL) |
| | 6 | |
| | 7 | == Meeting Notes == |
| | 8 | |
| | 9 | Plan for PoPLaR |
| | 10 | 1. Next SCAPA beamtime |
| | 11 | - Dates: July/August |
| | 12 | - Determine a baseline: |
| | 13 | - A baseline of repeating phase 2 cell irradiations was set |
| | 14 | |
| | 15 | 2. Aim for October |
| | 16 | - Paper |
| | 17 | - Aim for a physics publication with reference to the biology results |
| | 18 | - Add to Phase 3 plan: |
| | 19 | - Comet analysis provides DNA damage results and relatively easy to complete but requires more cell irradiations |
| | 20 | - Neutral and alkaline analyses seem the best to study single- and double-strand breaks |
| | 21 | - Also see the variation across the cell dish |
| | 22 | - Compare this and the clonogenics to the cyclotron irradiation |
| | 23 | - Questions to answer: |
| | 24 | - Is the spatial variation acceptable? |
| | 25 | |
| | 26 | 3. Aim for Post-October |
| | 27 | - Desired work/papers |
| | 28 | - Include IF but it is unnecessary to do in Phase 3 due to the additional irradiations required |
| | 29 | - X-ray comparison: Feasible to be included in Phase 3 |
| | 30 | - Would reproduce the same irradiation conditions (time out of incubator and vertical irradiation) as laser-driven protons |
| | 31 | - What do we need to know before we can plan more |
| | 32 | - Pre-proton electrons: |
| | 33 | - Could explore using the dipoles to remove these |
| | 34 | - Have finance for the dipole but need stands so unlikely to be Phase 3 |
| | 35 | - LET measurement |
| | 36 | - LET detector researched but unknown if it will be available for Phase 3 |
| | 37 | |
| | 38 | 4. ELI |
| | 39 | - Almost certainly post-October and ran out of time to properly discuss how this can be included in the plan. |
| | 40 | |
| | 41 | 5. Review steps required |
| | 42 | - Plan on Google Doc: https://docs.google.com/document/d/1OTARVtxhLRu02S6CLdRDB2QlGvCqDfZ3aWBifjIFs_Q/edit?usp=sharing |
| | 43 | - Steps on Trello: https://trello.com/invite/b/67587c515b0c69656ee78b48/ATTIe86f94b24e3e5884e4cb87e5ed3b89ab64321B83/poplar |
| | 44 | - Updates: |
| | 45 | - Diagnostics: |
| | 46 | - RCF: |
| | 47 | - Tony is delaminating some RCF and will compare with the delaminated EBT3 Mark supplied. |
| | 48 | - If we provide a batch we will use at SCAPA Tony can test this as well and do the calibration. |
| | 49 | - Suggestion that X-ray calibration removes the high-LET saturation effect. |
| | 50 | - Marie has offered to do the RCF calibration prior to travelling up |
| | 51 | - SciFi: |
| | 52 | - Peter has found a source for hollow fibre. |
| | 53 | - Cost: £300-400 for 15m, though Peter has money that can be put towards that. |
| | 54 | - Could do with a simulation to provide an order of magnitude estimate on the energy deposited on transport fibres |
| | 55 | - Tony has offered Birmingham to test the fibres before SCAPA |
| | 56 | - Make a package of everything we need and take to Birmingham |
| | 57 | - Also, would be able to test using a fibre around the cell dish here |
| | 58 | - Keep this simple at the start and just use one fibre |
| | 59 | - Bio: |
| | 60 | - Could potentially borrow Marie's inverted microscope but depends on other lab users |
| | 61 | - Marie is keen to meet up with Jason and Emma to help organise a dry run or testing on the control cell survival |
| | 62 | |
| | 63 | 6. DoNM |
| | 64 | - 21/05/26? |
| | 65 | |
| | 66 | 7. AoB |
| | 67 | - IPAC submitted |
| | 68 | |
| | 69 | === Summary of actions required === |
| | 70 | |
| | 71 | In-Beam Diagnostics |
| | 72 | - **CD, TP**: Refine evaluations of LET in the cells with the RCF in front |
| | 73 | - **DONE** |
| | 74 | - **CD**: Predict the cell dish dose from the RCF in front of it |
| | 75 | - **DONE** |
| | 76 | - **PH, CD**: Initial measurements of sci-fi arrays |
| | 77 | - **DONE** |
| | 78 | - **CD/KL**: Provide plan for Master's students who will count photons from UV source for sci-fi arrays |
| | 79 | - **DONE** |
| | 80 | - **Unassigned**: Acquire a glass sheet |
| | 81 | - **Unassigned**: Get a new RCF batch and calibrate |
| | 82 | - **PH**: Investigate how to get the light out of the vacuum chamber |
| | 83 | - **CD**: Simulate energy deposited in transport fibres |
| | 84 | - **KL, CD, JMcG, PH**: Make a full scifi prototype |
| | 85 | - **KL, CD, JMcG, PH**: Discuss visit to Birmingham to test SciFi |
| | 86 | - **RW, CD**: Study correlation between laser diagnostics and mean dose |
| | 87 | - **Unassigned**: Investigate how a phosphor sheet could be incorporated into the design of the cell dish |
| | 88 | |
| | 89 | Bio Next Steps |
| | 90 | - **CD**: Gather ideas for improving cell survival |
| | 91 | - **DONE** |
| | 92 | - **EM, CD, JMcG**: Write up technical summary of Phase 2 |
| | 93 | - **EM**: Write up a biology plan for a 2 PMQ setup |
| | 94 | - **Unassigned**: Obtain an inverted microscope |
| | 95 | - **Unassigned**: Obtain multi-chamber haemocytometers (Might be able to use Birmingham's) |
| | 96 | - **MB, JP, MB**: Meet up to plan cell survival dry run |
| | 97 | - **EM*: Run controls to examine how long the cells can survive vertically |
| | 98 | |
| | 99 | Beamline Modelling |
| | 100 | - **CD**: Find LET for different energies in the cell dish |
| | 101 | - **DONE** |
| | 102 | - **RW**: Examine energy spectra variation |
| | 103 | - **RW, CD**: Source characterisation |
| | 104 | |
| | 105 | Other Beamlines |
| | 106 | - **EM**: Coordinate ELIMED bid |
| | 107 | - **DONE** |
| | 108 | - **JMcG**: Simulate BELLA |
| | 109 | - **JMcG**: Simulate ELI |
| | 110 | |
| | 111 | ---- |
