Changes between Version 6 and Version 7 of Research/LhARA/RadiationBiology/BMthdlgy/ImprvCllSrvvl

Timestamp:

Apr 16, 2026, 6:10:09 PM (6 weeks ago)

Author:

ccd24

Comment:

--

Research/LhARA/RadiationBiology/BMthdlgy/ImprvCllSrvvl

@@ -5 +5 @@
   - Current limits are that they can survive 20 minutes but not ~1.5 hours
  2. Being aware of the issue
- 3. Reducing the time between shots from 20s to maybe 1s, definitely to 5s
+ 3. Reducing the time between shots from 20s to 5s
+  - This would bring the irradiation time from roughly 16 mins to roughly 4 mins
  4. Not irradiating RCF in the same carousel
-  - A combination of these two would bring the irradiation time from roughly 16 mins to roughly 4 mins
+
 - Dealing with potential causes: Temperature, CO2, lack of media, weak cell line
  5. Heat the carousel in which the cells are placed
@@ -17 +18 @@
   - Requires testing the cells at Birmingham and repeating the experiments
   - Not certain it will work
+
 - Equipment factors
  9. Place a thermostat in the incubator to check that it is not too warm
@@ -30 +32 @@
  == Personal Thoughts ==
 There are two things we do not know. The fastest we can irradiate cells and the time cells can survive vertically. 
-I think if we do 2, 3, and 4, we can get the time the cells are vertical for to less than 20 minutes. However, other factors may cause these to change, such as needing to take more shots, so I think it would be sensible to test how long the cells can last vertically (1) and see how much leeway we have. If there is no time, then I would go to SCAPA and test 5 and 6. If time allows, might as well test 7 and 8, but this feels like it will take longer for the new lids and the subsequent work of the new cell line. 
+I think if we do 2, 3, and 4, we can get the time the cells are vertical for to near 30 minutes. I think Robbie estmated that the externals including the transport, time to rotate carousel, mounting time, lock-up/prep time and post-irradiation checks take between 15-25 minutes. 3 reduces the irradiation time from 16 to 4 minutes, and 4 would then reduce that to 2 minutes, so then the externals are the biggest factor. A conservative estimate puts the time between 20-30 minutes, so it is possible that 2,3 and 4 are all that are needed to deal with the cell survivability issue. 
 
-Either way, a dry run is completely necessary before more irradiations can be done. 
+However, other factors may cause these to change, such as needing to take more shots, so I think it would be sensible to test how long the cells can last vertically (1) and see how much leeway we have. 
 
-Finally, I would definitely install a thermostat in the incubator; a monitor from Robbie would be great, but essential. 
+If there is no leeway, that is when I would start considering 5,6,7 and 8 (probably in that order?). 
+
+Either way, a dry run is necessary before more irradiations can be done. 
+
+Finally, I would definitely install a thermostat in the incubator; a monitor from Robbie would be great, but not essential given Robbie's workload. 
 
 I do not know enough about whether to use Marie's lab.